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Desarrollo de un test in vitro para la evaluación de la maduración de monocitos a macrófagos como posible predictor de una respuesta pro inflamatoria en implantes mamarios
dc.contributor.advisor | Solarte David, Víctor Alfonso | |
dc.contributor.advisor | Becerra Bayona, Silvia Milena | |
dc.contributor.author | Alfonso Hernández, Andrés Felipe | |
dc.contributor.author | Toloza Martínez, Diego Andrés | |
dc.contributor.author | Muriel Albadan, Juan Camilo | |
dc.coverage.spatial | Bucaramanga (Santander, Colombia) | spa |
dc.coverage.temporal | 2022 | spa |
dc.date.accessioned | 2023-03-01T16:16:38Z | |
dc.date.available | 2023-03-01T16:16:38Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12749/19152 | |
dc.description.abstract | En la actualidad, la popularidad de la mamoplastia de aumento ha acrecentado hasta el punto en que es una de las intervenciones quirúrgicas con índole estético más realizadas a nivel global (Kontoes & Gounnaris, 2017). No obstante, el implante tras el proceso de cicatrización puede generar una cápsula fibrosa, la cual recubre el implante, provocando dolor significativo, lo que puede llevar a una reintervención (Headon et al., 2015) (Kzhyshkowska et al., 2015). Teniendo en cuenta que este proceso de cicatrización se lleva a cabo principalmente por una respuesta inmune, en la cual se asocia una fase inflamatoria. Por ende, resulta idóneo evaluar la respuesta inflamatoria que provocará el biomaterial para su uso en implantes mamarios. Sin embargo, actualmente los modelos que permiten estimar con precisión la respuesta inmune que pueden mediar la cicatrización de este tipo de implantes, son en su mayoría in vivo. El presente proyecto se centra en la realización de una prueba de concepto, con el fin de determinar si la maduración de monocitos a macrófagos in vitro podría ser utilizada como ensayo de tamizaje de la respuesta proinflamatoria en materiales para implantes mamarios. Para ello, se utilizó monocitos (línea celular THP-1), los cuales se incubaron con el biomaterial de interés con el fin de evaluar su maduración a macrófago como indicativo indirecto de una respuesta pro-inflamatoria. Esta diferenciación se evaluó, determinando el cambio morfológico de los monocitos asociado a estructuras específicas como el núcleo y citoplasma, para esto se usaron tinciones fluorescentes tales como DAPI y Rodamina. La morfología fue analizada principalmente asociando su cambio en la circularidad como evidencia de transformación, además de adherencia al plato de cultivo. Los resultados evidenciaron que las células tratadas con el implante no presentaron una diferencia significativa en el tamaño del núcleo con respecto al control y el tamaño del citoplasma y circularidad presentan diferencias estadísticas respecto a las CTPMA, estas características morfológicas impiden clasificar las CTI como macrófagos M0. Demostrando que el implante silicona utilizado es compatible con los monocitos dentro de un ambiente controlado. Puesto que, 5 no generó una respuesta inmune significativa, característica que se considera normal al usar un implante que se encuentra habilitado para su uso en personas. | spa |
dc.description.tableofcontents | Problema u Oportunidad Marco Teórico Estado del arte Metodología Resultados Y Análisis De Resultados Conclusiones Y Recomendaciones Referencias Anexos | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | * |
dc.title | Desarrollo de un test in vitro para la evaluación de la maduración de monocitos a macrófagos como posible predictor de una respuesta pro inflamatoria en implantes mamarios | spa |
dc.title.translated | Development of an in vitro test for the evaluation of monocyte maturation at macrophages as a possible predictor of a proinflammatory response in breast implants | spa |
dc.degree.name | Ingeniero Biomédico | spa |
dc.publisher.grantor | Universidad Autónoma de Bucaramanga UNAB | spa |
dc.rights.local | Abierto (Texto Completo) | spa |
dc.publisher.faculty | Facultad Ingeniería | spa |
dc.publisher.program | Pregrado Ingeniería Biomédica | spa |
dc.description.degreelevel | Pregrado | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | |
dc.type.local | Trabajo de Grado | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | |
dc.subject.keywords | Biomedical engineering | spa |
dc.subject.keywords | Engineering | spa |
dc.subject.keywords | Medical electronics | spa |
dc.subject.keywords | Biological physics | spa |
dc.subject.keywords | Bioengineering | spa |
dc.subject.keywords | Medical instruments and apparatus | spa |
dc.subject.keywords | Medicine | spa |
dc.subject.keywords | Biomedical | spa |
dc.subject.keywords | Clinical engineering | spa |
dc.subject.keywords | Mammoplasty | spa |
dc.subject.keywords | Capsular contracture in implants | spa |
dc.subject.keywords | In vitro | spa |
dc.subject.keywords | Fluorescence staining | spa |
dc.subject.keywords | Cell morphology | spa |
dc.subject.keywords | Cell activation | spa |
dc.subject.keywords | Breast implants | spa |
dc.subject.keywords | Monocytes | spa |
dc.subject.keywords | Macrophage | spa |
dc.identifier.instname | instname:Universidad Autónoma de Bucaramanga - UNAB | spa |
dc.identifier.reponame | reponame:Repositorio Institucional UNAB | spa |
dc.type.hasversion | info:eu-repo/semantics/acceptedVersion | |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
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dc.contributor.cvlac | Solarte David, Víctor Alfonso [0001329391] | spa |
dc.contributor.cvlac | Becerra Bayona, Silvia Milena [0001568861] | spa |
dc.contributor.googlescholar | Becerra Bayona, Silvia Milena [5wr21EQAAAAJ] | spa |
dc.contributor.orcid | Solarte David, Víctor Alfonso [0000-0002-9856-1484] | spa |
dc.contributor.orcid | Becerra Bayona, Silvia Milena [0000-0002-4499-5885] | spa |
dc.contributor.scopus | Becerra Bayona, Silvia Milena [36522328100] | spa |
dc.contributor.researchgate | Becerra Bayona, Silvia Milena [Silvia_Becerra-Bayona] | spa |
dc.subject.lemb | Ingeniería biomédica | spa |
dc.subject.lemb | Ingeniería | spa |
dc.subject.lemb | Biofísica | spa |
dc.subject.lemb | Bioingeniería | spa |
dc.subject.lemb | Medicina | spa |
dc.subject.lemb | Biomédica | spa |
dc.subject.lemb | Implantes mamarios | spa |
dc.subject.lemb | Monocitos | spa |
dc.subject.lemb | Macrófago | spa |
dc.identifier.repourl | repourl:https://repository.unab.edu.co | spa |
dc.description.abstractenglish | Currently, the popularity of augmentation mammoplasty has increased to the point where it is one of the most performed cosmetic surgeries globally (Kontoes & Gounnaris, 2017). However, the implant after the healing process can generate a fibrous capsule, which covers the implant, causing significant pain, which can lead to a reoperation (Headon et al., 2015) (Kzhyshkowska et al., 2015). Taking into account that this healing process is carried out mainly by an immune response, in which an inflammatory phase is associated. Therefore, it is ideal to evaluate the inflammatory response that the biomaterial will cause for its use in breast implants. However, currently the models that make it possible to accurately estimate the immune response that can mediate the healing of this type of implants are mostly in vivo. This project focuses on carrying out a proof of concept, in order to determine if the maturation of monocytes to macrophages in vitro could be used as a screening test for the proinflammatory response in materials for breast implants. For this, monocytes (THP-1 cell line) were used, which were incubated with the biomaterial of interest in order to evaluate their maturation into macrophages as an indirect indicator of a pro-inflammatory response. This differentiation was evaluated, determining the morphological change of monocytes associated with specific structures such as the nucleus and cytoplasm, for this, fluorescent stains such as DAPI and Rhodamine were used. The morphology was mainly analyzed associating its change in circularity as evidence of transformation, as well as adherence to the culture dish. The results showed that the cells treated with the implant did not present a significant difference in the size of the nucleus with respect to the control and the size of the cytoplasm and circularity present statistical differences with respect to the CTPMA, these morphological characteristics prevent classifying the ITC as M0 macrophages. Demonstrating that the silicone implant used is compatible with monocytes within a controlled environment. Since 5 did not generate a significant immune response, a characteristic that is considered normal when using an implant that is approved for use in people. | spa |
dc.subject.proposal | Ingeniería clínica | spa |
dc.subject.proposal | Electrónica médica | spa |
dc.subject.proposal | Instrumentos y aparatos médicos | spa |
dc.subject.proposal | Mamoplastia | spa |
dc.subject.proposal | Contractura capsular en implantes | spa |
dc.subject.proposal | Tinción de fluorescencia | spa |
dc.subject.proposal | Morfología celular | spa |
dc.subject.proposal | Activación celular | spa |
dc.type.redcol | http://purl.org/redcol/resource_type/TP | |
dc.rights.creativecommons | Atribución-NoComercial-SinDerivadas 2.5 Colombia | * |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
dc.relation.uriapolo | https://apolo.unab.edu.co/en/persons/v%C3%ADctor-alfonso-solarte-david | spa |
dc.contributor.apolounab | Solarte David, Víctor Alfonso [víctor-alfonso-solarte-david] | spa |
dc.contributor.apolounab | Becerra Bayona, Silvia Milena [silvia-milena-becerra-bayona] | spa |
dc.coverage.campus | UNAB Campus Bucaramanga | spa |
dc.description.learningmodality | Modalidad Presencial | spa |
dc.contributor.linkedin | Becerra Bayona, Silvia Milena [silvia-becerra-3174455a] |
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