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dc.contributor.advisorSolarte David, Víctor Alfonso
dc.contributor.advisorBecerra Bayona, Silvia Milena
dc.contributor.authorSierra Sánchez, Freddy Alexis
dc.contributor.authorBrito Lizarazo, Carlos David
dc.coverage.spatialColombiaspa
dc.date.accessioned2021-08-19T14:56:30Z
dc.date.available2021-08-19T14:56:30Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/20.500.12749/13895
dc.description.abstractLos hidrogeles de fibrina obtenidos a partir de plasma rico en plaquetas (PRP) han despertado gran interés en los últimos años, ya que han demostrado resultados favorables en la ingeniería de tejidos, debido a su alto contenido de factores de crecimiento, así como la posibilidad de permitir la fabricación de andamios naturales, similares a las matrices extracelulares, idóneos para la regeneración tisular. Sin embargo, sus características mecánicas no han sido caracterizadas plenamente, lo que puede representar un obstáculo para el uso de este material como andamio para la regeneración de un tejido en específico. Por esta razón, en este estudio se fabricó hidrogeles de fibrina a partir de PRP, bajo diferentes parámetros de concentración de PRP y tiempos de gelación del hidrogel, con el fin de realizar pruebas de compresión, y documentar sus características mecánicas. Se fabricaron hidrogeles con tres concentraciones, la metodología estándar para fabricar PRP se denominó “concentración 100%”, respecto a ésta, se fabricó a una menor y mayor concentración de PRP, 75% y 133% respectivamente (los porcentajes se usan como etiqueta y no se refiere a la concentración real); asimismo, se variaron los tiempos de gelación para cada concentración de hidrogel, 2, 4 y 24 horas. Como resultado se evidenció que los módulos de elasticidad de los hidrogeles varían entre 2 kPa y 35 kPa. Adicionalmente, se determinó que los hidrogeles de PRP tienen una relación de hinchamiento de 6 a 32 veces su peso seco, una relación de re-hinchamiento entre 3.5 y 7.2 veces su peso seco, y una tasa de degradación entre 14% y 47% por hora. Este estudio permite tener un punto de partida para adecuar los hidrogeles de PRP según el uso que se busque en el área de ingeniería de tejidos, con el fin de obtener hidrogeles biomiméticos al ajustar sus propiedades mecánicas a las que presentan los tejidos a regenerar.spa
dc.description.tableofcontentsProblema u oportunidad .................................................................................................................. 9 Introducción ................................................................................................................................ 9 Planteamiento del problema ......................................................................................................... 9 Justificación ............................................................................................................................... 12 Pregunta Problema .................................................................................................................... 13 Objetivo General ........................................................................................................................ 13 Objetivos específicos .................................................................................................................. 13 Marco teórico ................................................................................................................................ 14 Biomateriales ............................................................................................................................. 14 Andamios ................................................................................................................................... 14 Biocompatibilidad .................................................................................................................. 14 Biodegradabilidad .................................................................................................................. 15 Arquitectura del andamio ...................................................................................................... 15 Propiedades mecánicas............................................................................................................... 16 Relación de hinchamiento de los hidrogeles................................................................................ 17 Trasplantes autólogos................................................................................................................. 17 Plaquetas .................................................................................................................................... 17 Fibrina y fibrinógeno ................................................................................................................. 18 Hemostasia ................................................................................................................................. 19 Plasma rico en plaquetas (PRP) ................................................................................................. 20 Hidrogeles .................................................................................................................................. 21 Hidrogeles de fibrina a partir de PRP ........................................................................................ 22 Análogos del PRP ....................................................................................................................... 23 Estado del arte ............................................................................................................................... 25 Metodología ................................................................................................................................... 33 Obtención del PRP ..................................................................................................................... 33 Fabricación de hidrogeles de PRP .............................................................................................. 34 Caracterización mecánica de los hidrogeles ............................................................................ 35 Pruebas de hinchamiento ........................................................................................................... 35 Pruebas de re-hinchamiento ....................................................................................................... 36 Pruebas de degradación ............................................................................................................. 37 Análisis estadístico ..................................................................................................................... 37 Resultados y análisis ...................................................................................................................... 39 Resultados .................................................................................................................................. 39 Obtención del PRP ................................................................................................................. 39 Fabricación de los hidrogeles de PRP ..................................................................................... 40 Medición del peso y tamaño de los hidrogeles ......................................................................... 42 Pruebas de compresión ........................................................................................................... 44 Relación de hinchamiento ....................................................................................................... 48 Relación de re-hinchamiento .................................................................................................. 50 Tasa de degradación ............................................................................................................... 51 Análisis de Resultados ................................................................................................................ 53 Conclusiones y recomendaciones ................................................................................................... 59 Bibliografía .................................................................................................................................... 59 ANEXOS ....................................................................................................................................... 70spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/*
dc.titleCaracterización mecánica de hidrogeles de fibrina partir de plasma rico en plaquetas con potencial uso en el diseño de matrices biomiméticasspa
dc.title.translatedMechanical characterization of fibrin hydrogels from platelet-rich plasma with potential use in the design of biomimetic matricesspa
dc.degree.nameIngeniero Biomédicospa
dc.publisher.grantorUniversidad Autónoma de Bucaramanga UNABspa
dc.rights.localAbierto (Texto Completo)spa
dc.publisher.facultyFacultad Ingenieríaspa
dc.publisher.programPregrado Ingeniería Biomédicaspa
dc.description.degreelevelPregradospa
dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.localTrabajo de Gradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1f
dc.subject.keywordsBiomedical engineeringspa
dc.subject.keywordsEngineeringspa
dc.subject.keywordsMedical electronicsspa
dc.subject.keywordsBiological physicsspa
dc.subject.keywordsBioengineeringspa
dc.subject.keywordsMedical instruments and apparatusspa
dc.subject.keywordsMedicinespa
dc.subject.keywordsPlatelet-rich plasmaspa
dc.subject.keywordsHydrogelsspa
dc.subject.keywordsCompression modulusspa
dc.subject.keywordsBiomaterialsspa
dc.subject.keywordsTissue regenerationspa
dc.subject.keywordsFibrinspa
dc.subject.keywordsBlood clottingspa
dc.subject.keywordsBloodspa
dc.subject.keywordsBlood plasmaspa
dc.subject.keywordsClinical engineeringspa
dc.identifier.instnameinstname:Universidad Autónoma de Bucaramanga - UNABspa
dc.identifier.reponamereponame:Repositorio Institucional UNABspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
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dc.contributor.cvlacSolarte David, Víctor Alfonso [0001329391]spa
dc.contributor.cvlacBecerra Bayona, Silvia Milena [0001568861]spa
dc.contributor.googlescholarBecerra Bayona, Silvia Milena [5wr21EQAAAAJ&hl=es&oi=ao]spa
dc.contributor.orcidSolarte David, Víctor Alfonso [0000-0002-9856-1484]spa
dc.contributor.orcidBecerra Bayona, Silvia Milena [0000-0002-4499-5885]spa
dc.contributor.researchgateSolarte David, Víctor Alfonso [Victor-Solarte-David]spa
dc.contributor.researchgateBecerra Bayona, Silvia Milena [Silvia-Becerra-Bayona]spa
dc.subject.lembIngeniería biomédicaspa
dc.subject.lembIngenieríaspa
dc.subject.lembBiofísicaspa
dc.subject.lembBioingenieríaspa
dc.subject.lembMedicinaspa
dc.subject.lembCoagulación sanguíneaspa
dc.subject.lembSangrespa
dc.subject.lembPlasma sanguíneospa
dc.identifier.repourlrepourl:https://repository.unab.edu.cospa
dc.description.abstractenglishFibrin hydrogels obtained from platelet-rich plasma (PRP) have aroused great interest in recent years, as they have shown favorable results in tissue engineering, due to their high content of growth factors, as well as the possibility to allow the manufacture of natural scaffolds, similar to extracellular matrices, suitable for tissue regeneration. However, its mechanical characteristics have not been characterized. fully, which can represent an obstacle to the use of this material as a scaffold for the regeneration of a specific tissue. For this reason, in this study, fibrin hydrogels were manufactured from PRP, under different parameters of PRP concentration and hydrogel gelation times, in order to perform compression tests and document their mechanical characteristics. Hydrogels were manufactured with three concentrations, the standard methodology to manufacture PRP was called "100% concentration", with respect to this, it was manufactured at a lower and higher concentration of PRP, 75% and 133% respectively (the percentages are used as labels and does not refer to the actual concentration); likewise, the gelation times were varied for each hydrogel concentration, 2, 4 and 24 hours. As a result, it was evidenced that the modulus of elasticity of the hydrogels varies between 2 kPa and 35 kPa. Additionally, it was determined that PRP hydrogels have a swelling ratio of 6 to 32 times their dry weight, a re-swelling ratio between 3.5 and 7.2 times their dry weight, and a degradation rate between 14% and 47% per time. This study allows us to have a starting point to adapt the PRP hydrogels according to the use sought in the area of ​​tissue engineering, with in order to obtain biomimetic hydrogels by adjusting their mechanical properties to those of the tissues to be regenerated.spa
dc.subject.proposalIngeniería clínicaspa
dc.subject.proposalElectrónica médicaspa
dc.subject.proposalInstrumentos y aparatos médicosspa
dc.subject.proposalPlasma rico en plaquetasspa
dc.subject.proposalHidrogelesspa
dc.subject.proposalMódulo de compresiónspa
dc.subject.proposalBiomaterialesspa
dc.subject.proposalRegeneración de tejidosspa
dc.subject.proposalFibrinaspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TP
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 2.5 Colombia*


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Atribución-NoComercial-SinDerivadas 2.5 Colombia
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 2.5 Colombia