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dc.contributor.authorBárcenas Cuadros, Claudia Maritzaspa
dc.contributor.authorCano, Luz Elenaspa
dc.contributor.authorCoock, Ana Maríaspa
dc.contributor.authorMartínez Pérez, Adrianaspa
dc.contributor.authorRestrepo Molina, Ángelaspa
dc.date.accessioned2020-10-27T14:22:06Z
dc.date.available2020-10-27T14:22:06Z
dc.date.issued2004-04-08
dc.identifier.issn2382-4603
dc.identifier.issn0123-7047
dc.identifier.urihttp://hdl.handle.net/20.500.12749/10486
dc.description.abstractAntecedentes: La paracoccidioidomicosis es una micosis pro-funda que se caracteriza por inflamación granulomatosa crónica que progresa a fibrosis pulmonar como resultado de falta de balance entre la síntesis y degradación de la matriz extracelular, pero los mecanismos implicados no se entienden claramente. Objetivo: Determinar en un modelo murino de fibrosis pulmonar inducido por P. brasiliensis, la expresión de colagenasa intersticial (MMP-1), gelatinasa A (MMP-2) y TIMP-1. Métodos: Tejido pulmonar obtenido por biopsia de 15 ratones BALB/c infectados con P. brasiliensis y ocho inoculados con solución salina se estudiaron a las se-manas 1, 4, 8, 12 y 16 postinfección. Resultados: A las 4 semanas del inúculo el 85,7% de los ratones tenían marcación para MMP-1 y MMP-2, y en el 71.4% para TIMP-1, todos de intensidad moderada en células epiteliales de los alveolos, mácrofagos alveolares y células de músculo liso alrededor de bronquíolos y vasos sanguíneos. A las 16 semanas en la gran mayoría de biopsias se observó una tinción moderada para estas metaloproteinas, aunque de mayor intensidad en la tercera parte de las muestras; la localización de estuvo en células epiteliales y mácrofagos alveolares. Conclusión: A mayor depósito de colágeno hay mayor disbalance de las metaloproteinas y sus inhibidores de tejidos, teniendo como patrón la producción y depósito secundario a una respuesta inflamatoria. Los macrófagos alveolares y las células interticiales alveolares son las principales fuentes celulares de producción de MMP-1, MMP-2 y TIMP-1. [Bárcenas CM, Cano LE, Coock AM, Martínez A, Restrepo A. Expresión de metaloproteinas y sus inhibidores de tejido en un modelo murino de fibrosis pulmonar.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad Autónoma de Bucaramanga UNAB
dc.relationhttps://revistas.unab.edu.co/index.php/medunab/article/view/237/220
dc.relation.urihttps://revistas.unab.edu.co/index.php/medunab/article/view/237
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.sourceMedUNAB; Vol. 7 Núm. 19 (2004): Fiebre amarilla, Fibrosis Pulmonar, Dislipidemia en diabetes; 9-14
dc.subjectCiencias biomédicas
dc.subjectCiencias de la vida
dc.subjectInnovaciones en salud
dc.subjectInvestigaciones
dc.titleExpresión de metaloproteinas y sus inhibidores de tejido en un modelo murino de fibrosis pulmonarspa
dc.title.translatedExpression of metalloproteins and their tissue inhibitors in a murine model of pulmonary fibrosisspa
dc.publisher.facultyFacultad Ciencias de la Saludspa
dc.publisher.programPregrado Medicinaspa
dc.type.driverinfo:eu-repo/semantics/article
dc.type.localArtículospa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501
dc.subject.keywordsHealth Scienceseng
dc.subject.keywordsMedicineeng
dc.subject.keywordsMedical Scienceseng
dc.subject.keywordsBiomedical Scienceseng
dc.subject.keywordsLife Scienceseng
dc.subject.keywordsInnovations in healtheng
dc.subject.keywordsResearcheng
dc.subject.keywordsPulmonary fibrosiseng
dc.subject.keywordsParacoccidioidomycosiseng
dc.subject.keywordsExtracellular matrixeng
dc.subject.keywordsMetalloproteinase 1 and 2eng
dc.subject.keywordsTissue inhibitor of metalloproteinaseeng
dc.identifier.instnameinstname:Universidad Autónoma de Bucaramanga UNABspa
dc.type.hasversionInfo:eu-repo/semantics/publishedVersion
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.relation.referencesEmonard H, Grimoud JA. Matriz metalloproteinases. A review. Cel Mol Biol 1990; 36:131-53spa
dc.relation.referencesBrummer E, Castañeda E, Restrepo A. Paracoccidioidomycosis an update. Clin Microb Rew 1993; 6:89-117spa
dc.relation.referencesBrito T, Franco MF. Granulomatous inflammation. Rev Inst Med Trop Sao Paulo 1994; 36:185-92.spa
dc.relation.referencesMcEwen JG, Bedoya V, Patiño ME, Salazar M, Restrepo A. Experimental murine paracoccidioidomycosis induced by the inhalation of conidia. J Med Vet Mycology 1987; 25:165-75spa
dc.relation.referencesRestrepo S, Tobón AM, Restrepo A. Development of pulmonary fibrosis in mice during infection with Paracoccidioides brasiliensis conidia. J Med Vet Mycol 1992; 30:173-84spa
dc.relation.referencesFranco L, Najvar L, Gómez B, Restrepo S, Restrepo A, Graybill JR. Experimental pulmonary fibrosis induced by Paracoccidioi-des brasiliensis conidia: Measurement of the host local respon-ses. Am J Trop Med Hyg 1998; 58:424-30.spa
dc.relation.referencesPerez-Ramos J, Segura ML, Vanda B, Pardo A. Matrix mellopro-teinases 2, 9, and 13, and tissue inhibitors of metalloproteinases 1 and 2 in experimental lung silicosis. Am J Respir Crit Care 1999; 160:1274-82.spa
dc.relation.referencesWoessner JF. The family of matrix metalloproteinases. Ann NY Acad Sci 1994; 732:11-21spa
dc.relation.referencesMadtes DK, Elston AL, Clark JG. Selective induction of tissue inhibitor of metalloproteinase-1 in bleomycin-induced pulmonary fibrosis. Am J Respir Cell Mol Biol 2001; 24:599-607spa
dc.relation.referencesRestrepo BI, McEwen JG, Salazar ME, Restrepo A. Morphoge-nesis of the conidia produced by P. brasiliensis mycelial form. J Med Vet Mycol 1986; 24:337-9spa
dc.relation.referencesSantana A, Saxena B, Noble NA, Gold LI, Marshall BC. Increa-sed expression of transforming growth factor B isoforms (b1, b2, b3) in bleomicin-induced pulmonar fibrosis. Am J Respir Cell Mol Biol 1995; 13:34-44spa
dc.relation.referencesObayashi Y, Yamadori I, Fujita J, Yoshinouchi T, Ueda N, Taka-hara J. The role of neutrophils in the pathogenesis of idiopatic pulmonary fibrosis. Chest 1997; 112:1338-44spa
dc.relation.referencesRoman J, Leon YJ, Gal A, Perez RL. Distribution of extracellular matrices, matrix receptors, and transforming growth factor-B1 in human and experimental lung granulomatous inflammation. Am J Med Sci 1995; 309:124-33.spa
dc.relation.referencesFukuda Y, Mochimaru H, Kudoh, S. Mechanism of structural remodeling in pulmonary fibrosis. Chest 120:41S-43Sspa
dc.contributor.googlescholarDfFAonMAAAAJ&hl=es&oi=ao [Martínez Pérez, Adriana]spa
dc.subject.lembCiencias médicasspa
dc.subject.lembCiencias de la saludspa
dc.subject.lembMedicinaspa
dc.identifier.repourlrepourl:https://repository.unab.edu.co
dc.description.abstractenglishBackground: Paracoccidioidomycosis is a deep mycosis characterized by chronic granulomatous inflammation that progresses to pulmonary fibrosis as a result of a lack of balance between the synthesis and degradation of the extracellular matrix, but the mechanisms involved are not clearly understood. Objective: To determine in a murine model of pulmonary fibrosis induced by P. brasiliensis, the expression of interstitial collagenase (MMP-1), gelatinase A (MMP-2) and TIMP-1. Methods: Lung tissue obtained by biopsy from 15 BALB/c mice infected with P. brasiliensis and eight inoculated with saline were studied at weeks 1, 4, 8, 12 and 16 postinfection. Results: At 4 weeks after inoculum, 85.7% of the mice had labeling for MMP-1 and MMP-2, and 71.4% for TIMP-1, all of moderate intensity in epithelial cells of the alveoli, alveolar macrophages. and smooth muscle cells around bronchioles and blood vessels. At 16 weeks, in the vast majority of biopsies, moderate staining for these metalloproteins was observed, although of greater intensity in a third of the samples; The localization was in epithelial cells and alveolar macrophages. Conclusion: The greater the collagen deposit, the greater the imbalance of metalloproteins and their tissue inhibitors, with the pattern being production and deposition secondary to an inflammatory response. Alveolar macrophages and alveolar interstitial cells are the main cellular sources of production of MMP-1, MMP-2 and TIMP-1. [Bárcenas CM, Cano LE, Coock AM, Martínez A, Restrepo A. Expression of metalloproteins and their tissue inhibitors in a murine model of pulmonary fibrosiseng
dc.subject.proposalFibrosis pulmonarspa
dc.subject.proposalParacoccidioidomicosisspa
dc.subject.proposalMatriz extracelularspa
dc.subject.proposalMetaloproteinasa 1 y 2spa
dc.subject.proposalInhibidor de tejido de metaloproteinasaspa
dc.type.redcolhttp://purl.org/redcol/resource_type/ART
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 2.5 Colombia*


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