Mostrar el registro sencillo del ítem
Capacidad discriminatoria y concordancia entre el ELISA-F29 y la PCR en individuos con infección por T. cruzi
dc.contributor.author | Gómez Laiton, Edgar David | spa |
dc.contributor.author | Polo Ardila, Laura Andrea | spa |
dc.contributor.author | Castellanos Domínguez, Yeny Zulay | spa |
dc.contributor.author | Herrera Galindo, Victor Mauricio | spa |
dc.contributor.author | Villar Centeno, Juan Carlos | spa |
dc.date.accessioned | 2020-10-27T14:19:59Z | |
dc.date.available | 2020-10-27T14:19:59Z | |
dc.date.issued | 2015-04-12 | |
dc.identifier.issn | 2382-4603 | |
dc.identifier.issn | 0123-7047 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12749/10106 | |
dc.description.abstract | El diagnóstico de la infección por Trypanosoma cruzi (T. cruzi) se realiza rutinariamente mediante pruebas serológicas mientras que el empleo de métodos moleculares se encuentra aún en proceso de estandarización. Objetivo: Evaluar la capacidad discriminatoria y concordancia entre una prueba serológica y una molecular para determinar la infección por T. cruzi. Métodos: Se realizó Reacción en Cadena de la Polimerasa (PCR) y la prueba de ELISA-F29 en 95 muestras de participantes de la cohorte "Cardiovascular health investigation and collaboration countries of America to assess the markers and outcomes of Chagas disease " CHICAMOCHA. Se evaluó la capacidad discriminatoria del ELISA-F29 respecto al resultado de PCR mediante la estimación del área bajo la curva ROC. Se estimó la tasa de falsos positivos al 25% y sensibilidad al 75%. Se determinó la concordancia mediante kappa de Cohen. Resultados: Se realizaron pruebas de PCR en dos momentos diferentes en 95 individuos (edad media: 38 años; 64% hombres), con tasas de positividad entre 1.1% – 2.2% para los primers S35-S36 y entre 18.3% – 34.7% para los primers 121-122, respectivamente. La capacidad discriminatoria del ELISAF29 respecto a PCR fue 0.62 (IC95%: 0.53; 0.70) y tasa de falsos positivos del 56% (IC95%: 42; 70). El punto de corte óptimo para el cociente de absorbancia fue 2.53 (sensibilidad 59% y especificidad 60%). Para el primer 121-122 los niveles de acuerdo observado y kappas estimados fueron: 52.6% y 0.10 (IC95%: -0.08, 0.28) para la primera medición, 62.4% y 0.09 (IC95%: -0.09, 0.28) para la segunda medición y 57.5% y 0.13 (IC95%: 0.01, 0.26) al evaluar simultáneamente las dos mediciones. Conclusiones: Los resultados demuestran una baja concordancia evidenciada por los valores de kappa determinados en el estudio. Es necesario afinar los estudios para evaluar la utilidad de las pruebas moleculares en el diagnóstico de la Enfermedad de Chagas. [Gómez-Laitón ED, Polo-Ardila LA, Castellanos-DomÍnguez YZ, Herrera VM, Villar JC. Capacidad discriminatoria y concordancia entre el ELISA-F29 y la PCR en individuos con infección por T. cruzi. MedUNAB 2015; 18 (1): 27-33]. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.publisher | Universidad Autónoma de Bucaramanga UNAB | |
dc.relation | https://revistas.unab.edu.co/index.php/medunab/article/view/2220/2054 | |
dc.relation.uri | https://revistas.unab.edu.co/index.php/medunab/article/view/2220 | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | |
dc.source | MedUNAB; Vol. 18 Núm. 1 (2015): Abril - Julio de 2015: Enfermedad Chagas, Leucemia linfoide, Cuidadores familiares; 27-33 | |
dc.subject | Reacción en Cadena de la Polimerasa | |
dc.subject | Enfermedad de Chagas | |
dc.subject | Trypanosoma cruzi | |
dc.subject | Diagnóstico | |
dc.subject | Serología | |
dc.subject | Polymerase Chain Reaction | |
dc.subject | Chagas Disease | |
dc.subject | Diagnosis | |
dc.subject | Serology | |
dc.subject | Reação em Cadeia da Polimerase | |
dc.subject | Doença de Chagas | |
dc.title | Capacidad discriminatoria y concordancia entre el ELISA-F29 y la PCR en individuos con infección por T. cruzi | |
dc.title.translated | Discriminatory Power and Concordance between ELISA-F29 and PCR in Individuals with Infection due to T.cruzi | |
dc.publisher.faculty | Facultad Ciencias de la Salud | |
dc.publisher.program | Pregrado Medicina | |
dc.type.driver | info:eu-repo/semantics/article | |
dc.type.local | Artículo | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | |
dc.subject.keywords | Reacción en Cadena de la Polimerasa | eng |
dc.subject.keywords | Enfermedad de Chagas | eng |
dc.subject.keywords | Trypanosoma cruzi | eng |
dc.subject.keywords | Diagnóstico | eng |
dc.subject.keywords | Serología | eng |
dc.subject.keywords | Polymerase Chain Reaction | eng |
dc.subject.keywords | Chagas Disease | eng |
dc.subject.keywords | Diagnosis | eng |
dc.subject.keywords | Serology | eng |
dc.subject.keywords | Reação em Cadeia da Polimerase | eng |
dc.subject.keywords | Doença de Chagas | eng |
dc.subject.keywords | Polymerase chain reaction | |
dc.subject.keywords | Chagas disease | |
dc.subject.keywords | Trypanosoma cruzi | |
dc.subject.keywords | Diagnosis | |
dc.subject.keywords | Serology | |
dc.identifier.instname | instname:Universidad Autónoma de Bucaramanga UNAB | spa |
dc.type.hasversion | Info:eu-repo/semantics/publishedVersion | |
dc.type.hasversion | info:eu-repo/semantics/acceptedVersion | |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.relation.references | WorldHealthOrganization.Researchprioritiesfor Chagas disease, human African trypanosomiasis and leishmaniasis.WorldHealOrganTechRepSer. 2012;975(v-xii):1–100 | |
dc.relation.references | Schofield CJ, Jannin J, Salvatella R. The future of Chagasdiseasecontrol.TrendsParasitol.2006 Dec;22(12):583–8 | |
dc.relation.references | Anthony R, Johnson C, Sousa O. Use of micro-ELISAfor quintitantingantibodytoTrypanosomacruziand Trypanosomarangeli.AmJTropHyg. 1979;28(6):969–73 | |
dc.relation.references | De Meis J, Morrot A, Farias-de-Oliveira DA, Villa-Verde DM, Savino W. Differential regional immune response in Chagas disease. PLoS Negl Trop Dis. 2009;3(7):e417 | |
dc.relation.references | Pavía PX, Roa NL, Uribe AMPC. Using S35-S36 and TcH2AF-Rprimer-basedPCRteststofollow-upa Chagas ́ disease patient who had undergone a heart transplant. Biomédica. 2011;31(2):178–84 | |
dc.relation.references | Barrera YK, Guevara JM, Pavía PX, Montilla M, Nicholls RS, Parra E, Puerta C. Evaluation of TcH2AF-R and S35-S36primersinPCRtestsforthedetectionof Trypanosoma cruzi in mouse cardiac tissue. Biomédica. 2008;28(4):616–26 | |
dc.relation.references | Moser DR, Kirchhoff LV, Donelson JE. Detection of Trypanosoma cruzi by DNAAmplification Using the 32Vol. 18(1):27-33, Abril - Julio 2015Edgar David Gómez-Laitón, Laura Andrea Polo-Ardila, Yeny Zulay Castellanos-Domínguez, Víctor Mauricio Herrera-Galindo, Juan Carlos Villar-Centeno 33PolymeraseChainReaction.JClinMicrobiol. 1989;27(7):1477–82 | |
dc.relation.references | Duffy T, Bisio M, Altcheh J, Burgos JM, Diez M, Levin MJ, et al. Accurate real-time PCRstrategy for monitoring bloodstream parasitic loads in chagas disease patients. PLoS Negl Trop Dis. 2009;3(4). | |
dc.relation.references | Schijman AG, Bisio M, Orellana L, Sued M, Duffy T, Mejia Jaramillo AM, et al. International study to evaluate PCRmethods for detection of Trypanosoma cruzi DNAin blood samples from Chagas disease patients. PLoS Negl Trop Dis. 2011;5(1) | |
dc.relation.references | Cannova D, Aguilar M, Pacheco M, Simons M, Medina M. Validación del Inmunoensayo enzimático (ELISA) y hemaglutinación indirecta (HAI) para el serodiagnóstico de la enfermedad e Chagas. Salus. 2002;6:4–9 | |
dc.relation.references | Da Silveira J, Umezawa E, Luguetti AO. Chagas disease: recombinant Trypanosoma cruzi antigens for serological diagnosis. TRENDSParasitol. 2001;17(6):286–91 | |
dc.relation.references | Afonso AM, Ebell MH, Tarleton RL. ASystematic Review of High Quality Diagnostic Tests for Chagas Disease. PLoS Negl Trop Dis. 2012;6(11) | |
dc.relation.references | Fabbro D, Velázquez E, Biza L, Denner S, Oliveira V et al. Evaluation of the ELISA-F29 test as an early marker of therapeuticefficacyinadultswithchronicChagas disease.RevInstMedTropSaoPaulo. 2013;55(3):167–72 | |
dc.relation.references | Bianchi F, Cucunubá Z, Guhl F, González NL, Freilij H, Nicholls RS, et al. Follow-up of an Asymptomatic Chagas Disease Population of Children after Treatment with Nifurtimox (Lampit) in a Sylvatic Endemic Transmission Area of Colombia. PLoS, Neglected Trop Dis [revista en internet].2015;10(2):e0003465.Disponibleen: http://dx.plos.org/10.1371/journal.pntd.0003465 | |
dc.relation.references | Gilbet SR, Alban SM, Gobor L, De Oliveira Bescrovaine J,MyiazakiMI,Thomaz-SoccolV.Comparisonof conventional serology and PCRmethods for the routine diagnosis of Trypanosoma cruzi infection. Rev Soc Bras Med Trop. 2013;46(April):310–5 | |
dc.relation.references | Ramírez JD, Guhl F, Umezawa ES, Morillo CA, Rosas F, Marín-Neto JA, et al. Evaluation of adult chronic Chagas' heart disease diagnosis by molecular and serological methods.JClinMicrobiol[revistaeninternet]. 2009;47(12):3945–51.Disponibleen: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2786654&tool=pmcentrez&rendertype=abstract | |
dc.relation.references | DuarteLF,FlórezO,RincónG,GonzálezCI. Comparisonofsevendiagnosticteststodetect Trypanosoma cruzi infection in patients in chronic phase of Chagas disease. Colombia Médica. 2014;2014:61–6 | |
dc.relation.references | Brasil PEa a, De Castro L, Hasslocher-Moreno AM, SangenisLHC,BragaJU.ELISAversusPCRfor diagnosis of chronic Chagas disease: systematic review and meta-analysis. BMCInfect Dis [revista en internet]. BioMedCentralLtd;2010;10(1):337.Disponibleen: http://www.biomedcentral.com/1471-2334/10/337 | |
dc.relation.references | De Winne K, Büscher P, Luquetti AO, Tavares SBN, Oliveira R a., Solari A, et al. The Trypanosoma cruzi SatelliteDNAOligoC-TesT andTrypanosomacruzi Kinetoplast DNAOligoC-TesTfor Diagnosis of Chagas Disease: AMulti-cohort Comparative Evaluation Study. PLoS Negl Trop Dis. 2014;8(1):55 | |
dc.contributor.cvlac | Villar Centeno, Juan Carlos [0000068519] | |
dc.contributor.googlescholar | Gómez Laiton, Edgar David [gUVSz4oAAAAJ&hl=es&oi=ao] | |
dc.contributor.googlescholar | Villar Centeno, Juan Carlos [nTlsWe0AAAAJ] | |
dc.contributor.orcid | Villar Centeno, Juan Carlos [0000-0002-7047-7299] | |
dc.subject.lemb | Patología | |
dc.subject.lemb | Medicina preventiva | |
dc.identifier.repourl | repourl:https://repository.unab.edu.co | |
dc.description.abstractenglish | The diagnosis of infection with Trypanosoma cruzi (T. cruzi) is routinely performed by serological tests while the use of molecular methods is still in process of standardization. Objective: To evaluate the discriminatory capacity and agreement between a serological test and a polymerase chain reaction (PCR) to determine T. cruzi infection. Methodology: PCR and ELISA test-F29 were performed to 95 participants of "Cardiovascular health investigation and collaboration countries of America to assess the markers and outcomes of Chagas disease " (CHICAMOCHA). Discriminatory capacity of ELISA –F29 with respect to PCR results were evaluated by estimating the area of ROC curve. The false positive rate was estimated to 25% and sensitivity to 75%. The agreement was determined using Cohen's kappa. Results: PCR tests were performed at two different times in 95 individuals (mean age: 38; 64% male), with positivity rates between 1.1 to 2.2% for S35-S36 primers and from 18.3% to 34, 7% for primers 121-122, respectively. ELISA-F29 discriminatory capacity regarding PCR was 0.62 (95% CI: 0.53, 0.70). The false positive rate was 56% (95% CI: 42; 70). The optimal cutoff for absorbance ratio of ELISA-F29 was 2.53 (sensitivity 59%, specificity 60%). For the primers 121-122, levels of observed agreement and kappa estimates were 52.6% and 0.10 (95% CI: -0.08, 0.28) for the first measurement, 62.4% and 0.09 (95% CI: -0.09, 0.28) for the second measurement, and 57.5% and 0.13 (95% CI: 0.01, 0.26) for the two measurements simultaneously evaluated. Conclusions: The results show poor agreement evidenced by kappa values determined in the study. It is necessary to refine the studies to evaluate the utility of molecular testing in the diagnosis of Chagas disease.[Gómez-Laitón ED, Polo-Ardila LA, Castellanos-DomÍnguez YZ, Herrera VM, Villar JC. Discriminatory power and concordance between ELISA-F29 and PCR in individuals with infection due to T.cruzi. MedUNAB 2015; 18 (1): 27-33]. | eng |
dc.subject.proposal | Reacción en cadena de la polimerasa | |
dc.subject.proposal | Enfermedad de Chagas | |
dc.subject.proposal | Trypanosoma cruzi | |
dc.subject.proposal | Diagnóstico | |
dc.subject.proposal | Serología | |
dc.identifier.doi | 10.29375/01237047.2220 | |
dc.type.redcol | http://purl.org/redcol/resource_type/ART | |
dc.rights.creativecommons | Atribución-NoComercial-SinDerivadas 2.5 Colombia | * |
dc.contributor.researchgroup | Grupo de Investigación en Cardiología Preventiva | spa |
dc.contributor.researchgroup | Grupo de Investigaciones Clínicas | spa |